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Samanta, Saptadip
- Enhanced Production of α-Amylase from Bacillus licheniformis SKB 4
Abstract Views :398 |
PDF Views:3
Authors
Saptadip Samanta
1,
Sanjay Kar
2,
Pradeep Kumar Das Mohapatra
3,
Bikash Ranjan Pati
3,
Keshab Chandra Mondal
3
Affiliations
1 Department of Physiology, Midnapore College, Midnapore, Paschim Medinipur 721101, West Bengal, IN
2 Department of Botany, Midnapore College, Midnapore, Paschim Medinipur 721101, West Bengal, IN
3 Department of Microbiology, Vidyasagar University, Midnapore, 721102, West Bengal, IN
1 Department of Physiology, Midnapore College, Midnapore, Paschim Medinipur 721101, West Bengal, IN
2 Department of Botany, Midnapore College, Midnapore, Paschim Medinipur 721101, West Bengal, IN
3 Department of Microbiology, Vidyasagar University, Midnapore, 721102, West Bengal, IN
Source
Indian Science Cruiser, Vol 31, No 5 (2017), Pagination: 32-39Abstract
The effect of some inductive factors including incubation temperature, aeration, inorganic phosphate source, supplementation of amino acids and vitamins on production of extracellular amylase by Bacillus licheniformis SKB 4 was optimized in a submerged fermentation. The maximum production of enzyme was obtained in highly aerobic condition at 42°C, pH 6.5 and 24 h incubation period. 1%, v/v inoculumn concentration exerts best result for growth and enzyme production. Several chemical factors such as inorganic phosphate (KH2PO4; 0.3%), amino acids (0.5 mg %; glutamic acid, cysteine, isoleucine), vitamins (biotin, 50μg/ml; pyridoxine, 50μg/ml; riboflavin, 20μg/ml), metallic salt MgSO4 (1 mM) and surfactant Tween 80 (0.075% (v/v) have positive effect on amylase production. Among the raw food grains used for amylase production, soya bean seed favoured enzyme production and rate production is much higher (2.5 fold) than the normal culture medium, whereas cereals and other pulses have no effect on enzyme production within normal incubation time. Use of raw food grain as culture medium and optimization of certain factors are the most important part for production of amylase in industrial sector.Keywords
Inductive Factors of Amylase Production from Bacillus licheniformis SKB4.
References
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- An Overview on Systemic Effects of Carbon Tetrachloride (CCl4) Exposure
Abstract Views :334 |
PDF Views:4
Authors
Barsha Dassarma
1,
Subhajit Jana
1,
Subham Rakshit
1,
Dilip K. Nandi
2,
Somnath Gangopadhyay
3,
Saptadip Samanta
4
Affiliations
1 Department of Physiology, Midnapore College, Midnapore, 721101, West Bengal, IN
2 Department of Physiology and Nutrition, Raja N.L. Khan Women’s College, Midnapore, 721102, West Bengal, IN
3 Department of Physiology, Occupational Ergonomics Laboratory, University College of Science and Technology, University of Calcutta, 92 APC Road, Calcutta 700009, West Bengal, IN
4 Department of Physiology, Midnapore College, Midnapore, IN
1 Department of Physiology, Midnapore College, Midnapore, 721101, West Bengal, IN
2 Department of Physiology and Nutrition, Raja N.L. Khan Women’s College, Midnapore, 721102, West Bengal, IN
3 Department of Physiology, Occupational Ergonomics Laboratory, University College of Science and Technology, University of Calcutta, 92 APC Road, Calcutta 700009, West Bengal, IN
4 Department of Physiology, Midnapore College, Midnapore, IN
Source
Indian Science Cruiser, Vol 32, No 3 (2018), Pagination: 36-40Abstract
Carbon tetrachloride (CCl4) is an environmental toxin with the ability to induce successive multi organs damage after formation of trichloromethyl radical through biotransformation. The later molecule causes lipid peroxidation, generation of ROS, reduction of antioxidant enzymes, membrane damage and breakdown of cytoarchitecture. The most affected organs are liver kidney, lung and brain tissue. The healthy liver becomes fibrotic, patchy one; renal dysfunction along with nephritis, nephrosis are the common outcomes; degeneration of alveolar septa, and elastic fibres are also observed followed by breath shortening, coughing, airway obstruction, and inflammation; central nervous system attributes short term memory loss, dementia, apraxia, paraphasias, tremor, and convulsion. The review concludes that exposure of CCl4 induces the damage of several vital organs.Keywords
Carbon Tetrachloride, Lipid Peroxidation, Oxidative Stress, Organs Damage.References
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- Studies on Regulation of Xylanase Bio-Synthesis by Trichoderma reesei SAF3
Abstract Views :343 |
PDF Views:3
Authors
Affiliations
1 Department of Botany, Midnapore College, Midnapore, Paschim Medinipur, 721101, West Bengal, IN
2 PG Department of Botany, Ramananda College, Bishnupur, Bankura, 722122, West Bengal, IN
3 Department of Physiology, Midnapore College, Midnapore, Paschim Medinipur, 721101, West Bengal, IN
4 Department of Microbiology, Vidyasagar University, Paschim Medinipur 721102, West Bengal, IN
1 Department of Botany, Midnapore College, Midnapore, Paschim Medinipur, 721101, West Bengal, IN
2 PG Department of Botany, Ramananda College, Bishnupur, Bankura, 722122, West Bengal, IN
3 Department of Physiology, Midnapore College, Midnapore, Paschim Medinipur, 721101, West Bengal, IN
4 Department of Microbiology, Vidyasagar University, Paschim Medinipur 721102, West Bengal, IN
Source
Indian Science Cruiser, Vol 33, No 3 (2019), Pagination: 19-26Abstract
Microbial xylanases have an exciting potentiality and are being used extensively in different industries. In this study, regulation of xylanase bio-synthesis was examined in Trichoderma reesei SAF3. Xylan (1.0% w/v) showed the highest inductive effect. Glucose at concentration of 1% (w/v) was repressive for xylanase bio-synthesis when used in presence or absences of xylan; the repression was partially elevated by addition of cyclic adenosine monophosphate (cAMP, 5mM). The above findings suggested that xylan was the major inducer and the induction was energy dependent, mediated by second messenger system.Keywords
Xylanase, Regulation of Biosynthesis, cAMP, Trichoderma Reesei.References
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